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rabbit polyclonal antibody against mouse scd1  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc rabbit polyclonal antibody against mouse scd1
    List of primer sequences used for RT-qPCR.
    Rabbit Polyclonal Antibody Against Mouse Scd1, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 95/100, based on 140 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rabbit+polyclonal+antibody+against+mouse+scd1/pmc04237372-93-24-31?v=Cell+Signaling+Technology+Inc
    Average 95 stars, based on 140 article reviews
    rabbit polyclonal antibody against mouse scd1 - by Bioz Stars, 2026-07
    95/100 stars

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    1) Product Images from "Uromodulin Retention in Thick Ascending Limb of Henle's Loop Affects SCD1 in Neighboring Proximal Tubule: Renal Transcriptome Studies in Mouse Models of Uromodulin-Associated Kidney Disease"

    Article Title: Uromodulin Retention in Thick Ascending Limb of Henle's Loop Affects SCD1 in Neighboring Proximal Tubule: Renal Transcriptome Studies in Mouse Models of Uromodulin-Associated Kidney Disease

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0113125

    List of primer sequences used for RT-qPCR.
    Figure Legend Snippet: List of primer sequences used for RT-qPCR.

    Techniques Used:

    ( A ) SCD1 was detected in the cytoplasmic compartment selectively of proximal tubular cells (in the straight S3 segment). In the kidney of the homozygous Umod C93F mutant mouse, SCD1 appeared to be abundant in a larger fraction of proximal tubular cells compared to the kidney of the wild-type mouse, and the average staining intensity of SCD1 positive cells appeared to be more prominent. Age of mice analyzed: four months. Umod wt : wild-type mouse; Umod C93F : homozygous Umod C93F mutant mouse. Uromodulin immunohistochemistry enabled identification of TALH segments. Proximal tubule segment are morphologically characterized by luminal microvilli. Chromogen: DAB for SCD1, Vector RED for UMOD; nuclear staining: hemalum. ( B ) Protein abundance of SCD1 in whole kidney lysate of homozygous Umod mutant mice of both lines was increased compared to wild-type mice. Signal intensities of SCD1 were corrected for GAPDH signal intensities of the same PVDF-membrane. Mean of protein abundance of wild-type mice was set on a value of 1 [mean (wild-type) = 1]. One-way-ANOVA with Tukey's Multiple Comparison Post hoc Test: p vs. wild-type, **, p <0.01; ***, p<0.001. Age of mice analyzed: four months.
    Figure Legend Snippet: ( A ) SCD1 was detected in the cytoplasmic compartment selectively of proximal tubular cells (in the straight S3 segment). In the kidney of the homozygous Umod C93F mutant mouse, SCD1 appeared to be abundant in a larger fraction of proximal tubular cells compared to the kidney of the wild-type mouse, and the average staining intensity of SCD1 positive cells appeared to be more prominent. Age of mice analyzed: four months. Umod wt : wild-type mouse; Umod C93F : homozygous Umod C93F mutant mouse. Uromodulin immunohistochemistry enabled identification of TALH segments. Proximal tubule segment are morphologically characterized by luminal microvilli. Chromogen: DAB for SCD1, Vector RED for UMOD; nuclear staining: hemalum. ( B ) Protein abundance of SCD1 in whole kidney lysate of homozygous Umod mutant mice of both lines was increased compared to wild-type mice. Signal intensities of SCD1 were corrected for GAPDH signal intensities of the same PVDF-membrane. Mean of protein abundance of wild-type mice was set on a value of 1 [mean (wild-type) = 1]. One-way-ANOVA with Tukey's Multiple Comparison Post hoc Test: p vs. wild-type, **, p <0.01; ***, p<0.001. Age of mice analyzed: four months.

    Techniques Used: Mutagenesis, Staining, Immunohistochemistry, Plasmid Preparation, Quantitative Proteomics, Membrane, Comparison

    Increased Scd1 transcript abundance in kidneys of homozygous Slc12a1 I299T mutant mice compared to the kidneys of littermate controls were detected by RT-qPCR analysis. Data are shown as scatter dot plot with mean (n = 5 per group). Age of mice analyzed: three months. Student's t test: p vs. wild-type, *, p <0.05.
    Figure Legend Snippet: Increased Scd1 transcript abundance in kidneys of homozygous Slc12a1 I299T mutant mice compared to the kidneys of littermate controls were detected by RT-qPCR analysis. Data are shown as scatter dot plot with mean (n = 5 per group). Age of mice analyzed: three months. Student's t test: p vs. wild-type, *, p <0.05.

    Techniques Used: Mutagenesis, Quantitative RT-PCR



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    rabbit polyclonal antibody against mouse scd1  (Cell Signaling Technology Inc)
    95
    Cell Signaling Technology Inc rabbit polyclonal antibody against mouse scd1
    List of primer sequences used for RT-qPCR.
    Rabbit Polyclonal Antibody Against Mouse Scd1, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rabbit+polyclonal+antibody+against+mouse+scd1/pmc04237372-93-24-31?v=Cell+Signaling+Technology+Inc
    Average 95 stars, based on 1 article reviews
    rabbit polyclonal antibody against mouse scd1 - by Bioz Stars, 2026-07
    95/100 stars
    		  Buy from Supplier

    Image Search Results


    List of primer sequences used for RT-qPCR.

    Journal: PLoS ONE

    Article Title: Uromodulin Retention in Thick Ascending Limb of Henle's Loop Affects SCD1 in Neighboring Proximal Tubule: Renal Transcriptome Studies in Mouse Models of Uromodulin-Associated Kidney Disease

    doi: 10.1371/journal.pone.0113125

    Figure Lengend Snippet: List of primer sequences used for RT-qPCR.

    Article Snippet: The following primary antibodies were used: rat monoclonal antibody against mouse ANGPTL7 (clone 538401; R&D Systems), rabbit monoclonal antibody against GAPDH (#2118, Cell Signaling), rabbit polyclonal antibody against mouse SCD1 (#2438, Cell Signaling).

    Techniques:

    ( A ) SCD1 was detected in the cytoplasmic compartment selectively of proximal tubular cells (in the straight S3 segment). In the kidney of the homozygous Umod C93F mutant mouse, SCD1 appeared to be abundant in a larger fraction of proximal tubular cells compared to the kidney of the wild-type mouse, and the average staining intensity of SCD1 positive cells appeared to be more prominent. Age of mice analyzed: four months. Umod wt : wild-type mouse; Umod C93F : homozygous Umod C93F mutant mouse. Uromodulin immunohistochemistry enabled identification of TALH segments. Proximal tubule segment are morphologically characterized by luminal microvilli. Chromogen: DAB for SCD1, Vector RED for UMOD; nuclear staining: hemalum. ( B ) Protein abundance of SCD1 in whole kidney lysate of homozygous Umod mutant mice of both lines was increased compared to wild-type mice. Signal intensities of SCD1 were corrected for GAPDH signal intensities of the same PVDF-membrane. Mean of protein abundance of wild-type mice was set on a value of 1 [mean (wild-type) = 1]. One-way-ANOVA with Tukey's Multiple Comparison Post hoc Test: p vs. wild-type, **, p <0.01; ***, p<0.001. Age of mice analyzed: four months.

    Journal: PLoS ONE

    Article Title: Uromodulin Retention in Thick Ascending Limb of Henle's Loop Affects SCD1 in Neighboring Proximal Tubule: Renal Transcriptome Studies in Mouse Models of Uromodulin-Associated Kidney Disease

    doi: 10.1371/journal.pone.0113125

    Figure Lengend Snippet: ( A ) SCD1 was detected in the cytoplasmic compartment selectively of proximal tubular cells (in the straight S3 segment). In the kidney of the homozygous Umod C93F mutant mouse, SCD1 appeared to be abundant in a larger fraction of proximal tubular cells compared to the kidney of the wild-type mouse, and the average staining intensity of SCD1 positive cells appeared to be more prominent. Age of mice analyzed: four months. Umod wt : wild-type mouse; Umod C93F : homozygous Umod C93F mutant mouse. Uromodulin immunohistochemistry enabled identification of TALH segments. Proximal tubule segment are morphologically characterized by luminal microvilli. Chromogen: DAB for SCD1, Vector RED for UMOD; nuclear staining: hemalum. ( B ) Protein abundance of SCD1 in whole kidney lysate of homozygous Umod mutant mice of both lines was increased compared to wild-type mice. Signal intensities of SCD1 were corrected for GAPDH signal intensities of the same PVDF-membrane. Mean of protein abundance of wild-type mice was set on a value of 1 [mean (wild-type) = 1]. One-way-ANOVA with Tukey's Multiple Comparison Post hoc Test: p vs. wild-type, **, p <0.01; ***, p<0.001. Age of mice analyzed: four months.

    Article Snippet: The following primary antibodies were used: rat monoclonal antibody against mouse ANGPTL7 (clone 538401; R&D Systems), rabbit monoclonal antibody against GAPDH (#2118, Cell Signaling), rabbit polyclonal antibody against mouse SCD1 (#2438, Cell Signaling).

    Techniques: Mutagenesis, Staining, Immunohistochemistry, Plasmid Preparation, Quantitative Proteomics, Membrane, Comparison

    Increased Scd1 transcript abundance in kidneys of homozygous Slc12a1 I299T mutant mice compared to the kidneys of littermate controls were detected by RT-qPCR analysis. Data are shown as scatter dot plot with mean (n = 5 per group). Age of mice analyzed: three months. Student's t test: p vs. wild-type, *, p <0.05.

    Journal: PLoS ONE

    Article Title: Uromodulin Retention in Thick Ascending Limb of Henle's Loop Affects SCD1 in Neighboring Proximal Tubule: Renal Transcriptome Studies in Mouse Models of Uromodulin-Associated Kidney Disease

    doi: 10.1371/journal.pone.0113125

    Figure Lengend Snippet: Increased Scd1 transcript abundance in kidneys of homozygous Slc12a1 I299T mutant mice compared to the kidneys of littermate controls were detected by RT-qPCR analysis. Data are shown as scatter dot plot with mean (n = 5 per group). Age of mice analyzed: three months. Student's t test: p vs. wild-type, *, p <0.05.

    Article Snippet: The following primary antibodies were used: rat monoclonal antibody against mouse ANGPTL7 (clone 538401; R&D Systems), rabbit monoclonal antibody against GAPDH (#2118, Cell Signaling), rabbit polyclonal antibody against mouse SCD1 (#2438, Cell Signaling).

    Techniques: Mutagenesis, Quantitative RT-PCR